Updated December 5
th
, 2018
4
Figures
Figures should be numbered separately with Arabic
numerals in the order of occurrence in the text of the
manuscript. One- or two-column format figures are
preferred. When appropriate, figures should include error
bars. A description of the statistical treatment of error
analysis should be included in the figure or scheme
legend.
Figure lettering should be in a clear, sans-serif typeface
(for example, Helvetica); if possible, the same typeface in
approximately the same font size should be used for all
figures in a paper. Use symbol font for Greek letters. All
display items should be on a white background, and
should avoid excessive boxing, unnecessary colour,
spurious decorative effects (such as three-dimensional
'skyscraper' histograms) and highly pixelated computer
drawings. The vertical axis of histograms should not be
truncated to exaggerate small differences. Labelling must
be of sufficient size and contrast to be readable, even
after appropriate reduction. The thinnest lines in the final
figure should be no smaller than one point wide.
Reasonable requests to enlarge figures will be
considered, but editors will make the final decision on
figure size. Authors will see a proof of figures.
Figures divided into parts should be labelled with a lower-
case bold a, b, and so on, in the same type size as used
elsewhere in the figure. Lettering in figures should be in
lower-case type, with only the first letter of each label
capitalized. Units should have a single space between the
number and the unit, and follow SI nomenclature (for
example, ms rather than msec) or the nomenclature
common to a particular field. Thousands should be
separated by commas (1,000). Unusual units or
abbreviations should be spelled out in full or defined in
the legend. Scale bars should be used rather than
magnification factors, with the length of the bar defined in
the legend rather than on the bar itself. In legends, please
use visual cues rather than verbal explanations, such as
"open red triangles".
Authors are encouraged to consider the needs of
colourblind readers (a substantial minority of the male
population) when choosing colours for figures. Many
colourblind readers cannot interpret visuals that rely on
discrimination of green and red, for example. Thus, we
ask authors to recolor green-and-red heatmaps, graphs
and schematics for which colours are chosen arbitrarily.
Recoloring primary data, such as fluorescence or rainbow
pseudo-coloured images, to colour-safe combinations
such as green and magenta, turquoise and red, yellow
and blue or other accessible colour palettes is strongly
encouraged.
Unnecessary figures should be avoided: data presented
in small tables or histograms, for instance, can generally
be stated briefly in the text instead. Figures should not
contain more than one panel unless the parts are logically
connected; each panel of a multipart figure should be
sized so that the whole figure can be reduced by the
same amount and reproduced on the printed page at the
smallest size at which essential details are visible.
When a manuscript is accepted for publication, we will
ask for high-resolution figure files, possibly in a different
electronic format. This information will be included in the
acceptance letter. See below for details of digital image
production and submission.
Gene nomenclature
Authors should use approved nomenclature for gene
symbols, and use symbols rather than italicized full names
(Ttn, not titin). Please consult the appropriate
nomenclature databases for correct gene names and
symbols. A useful resource is Entrez Gene. Approved
human gene symbols are provided by HUGO Gene
Nomenclature Committee (HGNC). Approved mouse
symbols are provided by The Jackson Laboratory.
For proposed gene names that are not already approved,
please submit the gene symbols to the appropriate
nomenclature committees as soon as possible, as these
must be deposited and approved before publication of an
article.
Avoid listing multiple names of genes (or proteins)
separated by a slash, as in 'Oct4/Pou5f1', as this is
ambiguous (it could mean a ratio, a complex, alternative
names or different subunits). Use one name throughout
and include the other at first mention: 'Oct4 (also known
as Pou5f1)'.
Supplementary information
Supplementary information should be submitted with the
manuscript and will be sent to referees during peer
review. Supplementary information is not copy-edited, so
authors should ensure that it is clearly and succinctly
presented, and that the style and terminology conform
with the rest of the paper. The following guidelines detail
the creation, citation and submission of supplementary
information. Please note that modification of
supplementary information after the paper is published
requires a formal correction, so authors are encouraged to
check their supplementary information carefully before
submitting the final version.
Designate each item as Supplementary Table, Figure,
Video, Audio, Notes, Data, Discussion or Equations.
Number Supplementary Tables and Figures as, for
example, "Supplementary Table 1". This numbering
should be separate from that used in tables and figures
appearing in the main printed article. Supplementary
Notes should not be numbered and can have an optional
title. Please provide a title for Supplementary Tables and
a title and a caption for Supplementary Figures,
Supplementary Video and Supplementary Notes. The
latter should only be used in consultation with the editors
for specific elements best presented in Supplementary
Information, such as standalone descriptions related to
methods (for example algorithm description, compound
synthesis and characterization). Please note that
Supplementary Methods will no longer be allowed.
Refer to each piece of supplementary information at least
once within the text of the main article, at the appropriate
point(s). Be sure to include the word "Supplementary"
each time one is mentioned. Please do not refer to
individual panels of supplementary figures.
Please also ensure that, where relevant, the appropriate
reporting guidance checklist (e.g. CONSORT for
randomised controlled trials, PRISMA for systematic